PolyZymes Lab
PUBLICATIONS
2023
49- Rocío López-Domene, Silvia Vázquez-Díaz, Evgenii Modin, Ana Beloqui*, Aitziber L. Cortajarena*. "An emerging nanozyme class for à la carte enzymatic-like activities based on protein-metal nanocluster hybrids". Advanced Functional Materials. Accepted
48.- M. Heredero, A. Beloqui*. “Enzyme-Polymer Conjugates for Tuning, Enhancing, and Expanding Biocatalytic Activity”. ChemBioChem, 2023, 24, 4, e202200611 **Special Issue: ChemBioTalents** DOI: 10.1002/cbic.202200611
2022
47.- A. Rodriguez-Abetxuko, D. Sánchez-deAlcázar, A. Reifs, A. Beloqui*. “A Versatile Chemoenzymatic Nanoreactor that Mimics NAD(P)H Oxidase for the In Situ Regeneration of Cofactors”. Angewandte Chemie International Edition, 2022, DOI: 10.1002/ange.202206926
46.- D. Sánchez-deAlcázar, A. Rodriguez-Abetxuko, A. Beloqui*. “Metal-Organic Enzyme Nanogels as Nanointegrated Self-reporting Chemobiosensors”. ACS Applied Materials and Interfases, 2022, 14, 24, 27589-27598, DOI: 10.1021/acsami.2c04385
45.- M. S. Orellano, O. Sanz, S. Camarero-Espinosa, A. Beloqui, and M. Calderón*. “Recent advances and future perspectives of porous materials for biomedical applications”. Nanomedicine, 2022 17:4, 197-200. DOI: 10.2217/nnm-2021-0436
2020
44.- Nanoconfined (bio)catalysts as efficient glucose-responsive nanoreactors
Andoni Rodriguez-Abetxuko, Pablo Muñumer, Mitsuhiro Okuda, Javier Calvo, Mato Knez, Ana Beloqui. Advanced Functional Materials. DOI: 10.1002/adfm.202002990
43.- Hetero-Diels–Alder Cycloaddition with RAFT Polymers as Bioconjugation Platform
Ana Beloqui, Shivshankar R. Mane, Marcel Langer, Mathias Glassner, Dennis M. Bauer, Ljiljana Fruk, Christopher Barner-Kowollik,* and Guillaume Delaittre. Angewandte Chemie International Edition. DOI: 10.1002/anie.202005747
42.- Tunable polymeric scaffolds for enzyme immobilization
Andoni Rodriguez-Abetxuko, Daniel Sánchez de Alcázar, Pablo Muñumer, Ana Beloqui. Frontiers in Bioengineering and Biotechnology. DOI: 10.3389/fbioe.2020.00830
41.- Protein-based functional hybrid bionanomaterials by bottom-up approaches
A. Beloqui* and A. López-Cortajarena* (*: shared coauthorship). Current Opinion in Structureal Biology. DOI: 10.1016/j.sbi.2020.04.005
40.- Making the Best of It: Nitroxide-Mediated Polymerization of Methacrylates via the Copolymerization Approach with Functional Styrenics
Aaron C. Schmidt, Hatice Turgut, Dao Le, Ana Beloqui and Guillaume Delaittre. Polymer Chemistry. DOI:10.1039/C9PY01458F
2019
39.- A Versatile Approach for the Assembly of Highly Tunable Biocatalytic Thin Films
A. Rodriguez‐Abetxuko, D. Sánchez‐deAlcázar, A. L. Cortajarena, A. Beloqui. Advanced Materials Interfaces. DOI: 10.1002/admi.201900598
38.- Carrierless Immobilization Route for Highly Robust Metal–Organic Hybrid Enzymes
A. Rodriguez‐Abetxuko, M-C. Morant Miñana, M. Knez, A. Beloqui. ACS Omega. DOI: 10.1021/acsomega.8b03559
37.- Supramolecular assembly of the benzimidazole derivatives
A. Beloqui. Chemistry and Applications of Benzimidazole and its Derivatives. DOI: 10.5772/intechopen.85333
2018
36.- Imidazole-Grafted Nanogels for the Fabrication of Organic-Inorganic Protein Hybrids
A. Rodriguez‐Abetxuko, M-C. Morant Miñana, L. Yate, M. Knez, A. Beloqui. Advanced Functional Materials. DOI: 10.1002/adfm.201803115
35.- Sugar stabilizes single-enzymes nanogels
M. Doug. Highlight article of "A simple route to highly active single-enzyme nanogels" in MRS Bulletin.
34.- A simple route to highly active single-enzyme nanogels
A. Beloqui, A.-Y. Kobitski, G.U. Nienhaus, G. Delaittre. Chemical Science. DOI: 10.1039/c7sc04438k
33.- Controlled manipulation of enzyme specificity through immobilization-induced flexibility constraints
C. Coscolín, A. Beloqui, M. Martínez-Martínez, R. Bargiela, G. Santiago, R. M. Blanco, G. Delaittre, C. Márquez-Álvarez, M. Ferrer. Applied Catalysis A: General. DOI: 10.1016/j.apcata.2018.08.003
32.- Impact of Polymer Bioconjugation on Protein Stability and Activity Investigated with Discrete Conjugates: Alternatives to PEGylation
J. Morgenstern, G. Gil-Alvaradejo, N. Bluthardt, A. Beloqui, G. Delaittre, J. Hubbuch. Biomacromolecules. DOI: 10.1021/acs.biomac.8b01020
2017
31.-Nitrilotriacetic Amine-Functionalized Polymeric Core-Shell Nanoparticles as Enzyme Immobilization Supports
D. Keller, A. Beloqui, M. Martínez-Martínez, M. Ferrer, G. Delaittre. Biomacromolecules. DOI: 10.1021/acs.biomac.7b00677
2016
30.- Single-Molecule Encapsulation: A Straightforward Route to Highly Stable and Printable Enzymes
A. Beloqui, S. Baur, V. Trouillet, A. Welle, J. Madsen, M. Bastmeyer, G. Delaittre. Small. DOI: 10.1002/smll.201503405
2015
29.- Algal lectin binding to core (alpha 1-6) fucosylated N-glycans: Structural basis for specificity and production of recombinant protein
A. do Nascimento, S. Serna, A. Beloqui, A.Arda, A.H Sampaio, J. Walcher, D. Ott, C. Unverzagt, N.-C. Reichardt, J. Jimenez-Barbero, K. Nascimento, A. Imberty, B.S Cavada, A. Varrot. Glycobiology. DOI: 10.1093/glycob/cwv002
28.- Nanostructured Indium Tin Oxide Slides for Small-Molecule Profiling and Imaging Mass Spectrometry of Metabolites by Surface-Assisted Laser Desorption Ionization MS
C. Lopez de Laorden†, A. Beloqui†, L. Yate, J. Calvo, M. Puigivila, J. Llop, N.-C. Reichardt. Analytical Chemistry. DOI: 10.1021/ac5025864
2013
27.- Analysis of Microarrays by MALDI-TOF MS
A. Beloqui, J. Calvo, S. Serna, S. Yan, I. B. H. Wilson, M. Martin-Lomas, N.-C. Reichardt. Angewandte Chemie International Edition. DOI: 10.1002/anie.201302455
26.- Genome sequence and functional genomic analysis of the oil-degrading bacterium Oleispira antarctica
M. Kube, T.N. Chernikova, Y. Al-Ramahi, A. Beloqui, ..., R. Reinhardt, P. N. Golyshin. Nature Communications. DOI: 10.1038/ncomms3156
25.- Three-Dimensional Arrays Using GlycoPEG Tags: Glycan Synthesis, Purification and Immobilisation
J. Etxebarria, S. Serna, A. Beloqui, M. Martin-Lomas, N.-C. Reichardt. Chemistry - A European Journal. DOI: 10.1002/chem.201204004
2012
24.- A surface-based mass spectrometry method for screening glycosidase specificity in environmental samples
A. Beloqui†, A. Sanchez-Ruiz†, M. Martin-Lomas, N.-C. Reichardt. Chemical Communications. DOI: 10.1039/c2cc16537f
23.- Functional Metagenomics Unveils a Multifunctional Glycosyl Hydrolase from the Family 43 Catalysing the Breakdown of Plant Polymers in the Calf Rumen
M. Ferrer†, A. Ghazi†, A. Beloqui†, J.M. Vieites, J. Martin-Navarro, T.Y. Nechitaylo, M.-E. Guazzaroni, J. Polaina, A. Waliczek, T.N. Chernikova, O.L. Reva, O.V. Golyshina, P.N. Golyshin. PLOS One. DOI: 10.1371/journal.pone.0038134
2011
22.- Microbes and Enzymes: Recent Trends and New Directions to Expand Protein Space
A. Beloqui, M. Zumarraga, M. Alcalde, P.N. Golyshin, M. Ferrer. Protein Engineering Handbook. DOI: 10.1002/9783527634026.ch9
2010
21.- Enzymatic functionalization of hydrocarbons-like molecules
N. Lopez-Cortes, A. Beloqui, A. Ghazi, M. Ferrer. Handbook of Hydrocarbon and Lipid Microbiology. DOI: 10.1007/978-3-540-77587-4_211
20.- Procedures for protein isolation in pure culture and microbial communities
A. Beloqui, M.-E. Guazzaroni, M. Ferrer. Handbook of Hydrocarbon and Lipid Microbiology. DOI: 10.1007/978-3-540-77587-4_326
19.- Metagenomic Mining of Enzyme Diversity
M.-E. Guazzaroni, A. Beloqui, J.M. Vieites, Y. Al-ramahi, N. Lopez-Cortes, A. Ghazi, P.N. Golyshin, M. Ferrer.
Handbook of Hydrocarbon and Lipid Microbiology. DOI: 10.1007/978-3-540-77587-4_216
18.- Diversity of Glycosyl Hydrolases from Cellulose-Depleting Communities Enriched from Casts of Two Earthworm Species
A. Beloqui, T. Y. Nechitaylo, N. López-Cortés, A. Ghazi, M.-E. Guazzaroni, J. Polaina, A. W. Strittmatter, O. Reva, A. Waliczek, M.M. Yakimov, O. V. Golyshina, M. Ferrer, P.N. Golyshin. Applied and Environmental Microbiology. DOI: 10.1128/AEM.00902-10
17.- Inter-conversion of catalytic abilities in a bifunctional carboxyl/feruloyl-esterase from earthworm gut metagenome
J. M. Vieites†, A. Ghazi†, A. Beloqui†, J. Polaina, J.M. Andreu, O.V. Golyshina, T.Y. Nechitaylo, A. Waliczek, M.M. Yakimov, P.N. Golyshin, M. Ferrer. Microbial Biotechnology. DOI: 10.1111/j.1751-7915.2009.00135.x
16.- Metagenomic era for biocatalyst identification
L. Fernández-Arrojo, M.-E. Guazzaroni, N. López-Cortés, A. Beloqui, M. Ferrer. Current Opinion in Biotechnology. DOI: 10.1016/j.copbio.2010.09.006
15.- Molecular Methods to Study Complex Microbial Communities
J.M. Vieites, M.-E. Guazzaroni, A. Beloqui, P.N. Golyshin, M. Ferrer. Methods in molecular biology . DOI: 10.1007/978-1-60761-823-2_1
14.- Novel Hybrid Esterase-Haloacid Dehalogenase Enzyme
A. Beloqui, J. Polaina, J.M. Vieites, D. Reyes-Duarte, R. Torres, O.V. Golyshina, T.N. Chernikova, A. Waliczek, A. Aharoni, M.M. Yakimov, K.N. Timmis, P.N. Golyshin, M. Ferrer. ChemBioChem. DOI: 10.1002/cbic.201000258
13.-Screening metagenomic libraries for laccase activities
M. Ferrer, A. Beloqui, P.N. Golyshin. Metagenomics: Methods and Protocols. DOI: 10.1007/978-1-60761-823-2_13
2009
12.- Interplay of metagenomics and in vitro compartmentalization
M. Ferrer, A. Beloqui, J.M. Vieites, M.-E. Guazzaroni, I. Berger, A. Aharoni. Microbial Biotechnology. DOI: 10.1111/j.1751-7915.2008.00057.x
11.- Metagenomics approaches in systems microbiology
J.M. Vieites, M.-E. Guazzaroni, A. Beloqui, P. N. Golyshin, M. Ferrer. FEMS Microbiology Reviews. DOI: 10.1111/j.1574-6976.2008.00152.x
10.- Metagenomics as a new technological tool to gain scientific knowledge
M.-E. Guazzaroni, A. Beloqui, P.N. Golyshin, M. Ferrer. World Journal of Microbiology and Biotechnology. DOI: 10.1007/s11274-009-9971-z
9.- Metagenomics for Mining New Genetic Resources of Microbial Communities
M. Ferrer, A. Beloqui, K.N. Timmis, ·P.N. Golyshin. Journal of Molecular Microbiology and Biotechnology.DOI: 10.1159/000142898
2008
8.- A purple acidophilic di-ferric DNA ligase from Ferroplasma
M. Ferrer, O. V. Golyshina, A. Beloqui, L.H. Böttger, J.M. Andreu, J. Polaina, A. L. De Lacey, A. X. Trautwein, K.N. Timmis, Peter N. Golyshin. Proceedings of the National Academy of Sciences of the United States of America (PNAS). DOI: 10.1073/pnas.0800071105
7.- Recent trends in industrial microbiology
A. Beloqui, P. Domínguez de María, P. N. Golyshin, M. Ferrer. Current Opinion in Microbiology. DOI: 10.1016/j.mib.2008.04.005
2007
6.- Biochemical and structural features of a novel cyclodextrinase from cow rumen metagenome
M. Ferrer, A. Beloqui, O.V. Golyshina, F.J. Plou, A. Neef, T.N. Chernikova, L. Fernandez-Arrojo, I. Ghazi, A. Ballesteros, K. Elborough, K.N. Timmis, P.N. Golyshin. Biotechnology Journal. DOI: 10.1002/biot.200600183
5.- Catalytic role of conserved HQGE motif in the CE6 carbohydrate esterase family
N. Lopez-Cortes, D. Reyes-Duarte, A. Beloqui, J. Polaina, I. Ghazi, O.V. Golyshina, A. Ballesteros, P.N. Golyshin, M. Ferrer. FEBS Letters. DOI: 10.1016/j.febslet.2007.08.060
4.- Microbial metagenomes: moving forward industrial biotechnology
M. Ferrer, A. Beloqui, P.N. Golyshin. Journal of Chemical Technology and Biotechnology. DOI: 10.1002/jctb.1695
3.- Mining enzymes from extreme environments
M. Ferrer, O.V. Golyshina, A. Beloqui, P.N. Golyshin. Current Opinion in Microbiology. DOI: 10.1016/j.mib.2007.05.004
2.- The cellular machinery of Ferroplasma acidiphilum is iron-protein-dominated
M. Ferrer, O.V. Golyshina, A. Beloqui, P.N. Golyshin, K.N. Timmis. Nature. DOI: 10.1038/nature05362
2006
1.- Novel polyphenol oxidase mined from a metagenome expression library of bovine rumen - Biochemical properties, structural analysis, and phylogenetic relationships
A. Beloqui, M. Pita, J. Polaina, A. Martinez-Arias, O.V. Golyshina, M. Zumarraga, M.M. Yakinov, H. Garcia-Arellano, M Alcalde, V.M. Fernandez, K. Elbororough, J.M. Andreu, A. Ballesteros, F.J. Plou, K.N. Timmis, M. Ferrer, P.N. Golyshin.
Journal of Biological Chemistry. DOI: 10.1074/jbc.M600577200